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ExoComplete 96-Wells Plate Kit





About ExoComplete

The ExoComplete system makes the exosomal mRNA isolated from fragments like fat in a 96 wells-plate. A significantly big amounts like urine are separated in a collection tube. This way Exosomes and microvesicles are left alone from body fluids and are caught with Exosome Isolation Plate or Collection Tube with a patented filter material. The highly absorbed material gives the possibillity of fast filtration of those fats with no hamper, and repeating isolation of Exosome Isolation Plates without the use of repetitive ultracentrifugation.

What ExoComplete offers:

  • One Kit, One Platform
  • Seamless Process Integration
  • High Throughput
  • Compatibility with Standard Automated Systems
  • Easy Use
  • Sample Versatility - Serum, Plasma, Urine, etc.

In addition, the separated Exosomes can be lysed immediately on the filter material by adding lysis buffer. Guanidine thiocyanate and other types of buffer directly exclude ribonucleases to ensure separation of intact mRNA. Antisense Primers of target genes are connected and added in the lysis buffer. The A. P. in the lysis buffer hybridize to the target mRNA and maximise the expertise of reverse transcription reaction.

After redirecting the Exosome Isolation Plate lysates to the mRNA plate, mRNA can be isolated with hybridization with single strand oligo(DT), immobilized in the wells of the mRNA capture plate. The plus of this method over conventional magnet beads and spin column methods is that oligonucleotides are struck on the surface of the well where mRNA hybridization happens.

The aftermath in higher mRNA income and better consistency between well to well and assay to assay runs, where to conventional fication method is less. Once the hybridization to the mRNA Capture Plate is complete, a wide choice of downstream applications come to help, including:

  • mRNA quantification
  • Single and double-stranded cDNA synthesis
  • RT-PCR
  • Real time quantitative RT-PCR (qPCR)
  • NGS RNA sequencing, etc.

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